PLASMID PROFILING AND CURING OF TYPHOID (SALMONELLA TYPHI) AND NON-TYPHOID (SALMONELLA ENTERITIDIS) ISOLATES FROM ENUGU, NIGERIA
Abstract
Medicine is currently grappling with the issue of antibiotic resistance in bacteria, which has arisen due to the rapid multiplication and widespread dissemination of these organisms, coupled with their ability to effortlessly acquire new genetic material. This work was carried out to profile and cure plasmid of typhoid and non-typhoid Salmonella using thirty-five (35) blood samples taken from students of Godfrey Okoye University. Several analyses such as widal test, morphological analysis, biochemical test, antibiotic sensitivity test, plasmid curing and plasmid profiling were performed. The results showed that among the fifteen isolates obtained that ten were identified as Salmonella typhi and five Samonella enteritidis. Notably, all isolates were resistant to sparfloxacin and cefotaxime, each demonstrating a 90% resistance rate with zones of inhibition less than 13 mm. In contrast, the isolates were susceptible to azithromycin (80%) and levofloxacin (70%), with zones of inhibition exceeding 25 mm. Plasmid curing was performed on eight bacterial isolates that exhibited resistance to more than four antibiotics, followed by a reassessment of their antibiotic sensitivity. Post-curing, these isolates remained highly resistant to sparfloxacin (75%), cefotaxime (62.5%), and augmentin (50%), each with zones of inhibition less than 14 mm. However, resistance to azithromycin and levofloxacin significantly decreased, with only 12.5% of the isolates remaining resistant, and zones of inhibition exceeding 30 mm for these antibiotics. The curing of plasmids resulted in a shift in antibiotic susceptibility for some isolates, where previously resistant isolates became susceptible, while others maintained their resistance. This change underscores the role of plasmids in conferring antibiotic resistance among the bacterial isolates. Molecular analysis revealed that the majority of isolates carried plasmids with high molecular weights ranging from 3kbp to 10kbp, with S2, S3, S5, and S12 losing their 3kbp plasmids and S11, S14, and N8 retaining theirs.
Keywords:
Widal test, Antibiotic resistance, plasmid curing, plasmid profilingDownloads
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Copyright (c) 2024 Ekwem C. C, Eze Emmanuel A, Ukwueze N. M
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References
Akinyemi, K. O., Smith, S. I., Oyefolu, A. O. and Coker, A. O. (2005). Multidrug resistance in Salmonella enterica serovar typhi isoslated from patients with typhoid fever complications in Lagos, Nigeria. Public Health. 119: 321–327.
Ali, M. H., Rokonuzzaman, S. M., Ahmed, M. A., Uddin, M. N., Haque, M. A. and Islam, M. A. (2011). Effectiveness of levofloxacin in enteric fever. Mymensingh Med J. 20(3): 441-5.
Aworh, M. K., Kwaga, J. K. P., Hendriksen, R. S., Okolocha, E. C. and Thakur, S. (2021). Genetic relatedness of multidrug resistant Escherichia coli isolated from humans, chickens and poultry environments. Antimicrobial Resistance and Infection Control. 10(1): 58.
Bennett, P. M. (2008). Plasmid encoded antibiotic resistance: acquisition and transfer of antibiotic resistance genes in bacteria. British Journal of Pharmacology. 153(1): 347–357.
Effa, E. E. and Bukirwa, H. (2011). Azithromycin for treating uncomplicated typhoid and paratyphoid fever (enteric fever). Cochrane Database Syst Rev. 2008 Oct 8; (4):CD006083. doi: 10.1002/14651858.CD006083.pub2. Update in: Cochrane Database Syst Rev. (10):CD006083. PMID: 18843701.
Ehiaghe, F. A., Ehiaghe, I. J., Agbonlahor, D. E., Oviasogie, F. E., Etikerentse, S. M. O., Nwobu, R. A. U., Akinshipe, B. O., Ikusemore, A. I., Aladenika, S. T. and Enwa, F. O. (2013). Plasmid Profiling and Curing Analysis of Fluoroquinolone Multidrug Resistant Pseudomonas aeruginosa in Benin City, Nigeria. Open Journal of Medical Microbiology. 3: 201-205.
Ekundayo, O. K. (2021). Plasmid Profile and Plasmid Curing of Some Enterobacteriaceae Resistant to Ampicillin Isolated from Food, Human Stool, and Chicken Stool in Ekiti State. Department of Biology, School of Science Bamidele Olumilua University of Education Science and Technology Ikere Ekiti, Ekiti State, Nigeria. Int. J. Curr. Microbiol. App. Sci. 10(1): 2344-2362.
Faisal, S. M. W., Alam, A. K. and Sajed, M. N. (2017). Study of antibiotic sensitivity pattern of Salmonella typhi and Salmonella paratyphi isolated from blood samples in Dhaka city. The Pharmacy innovation. 6(1): 93.
Feasey, N. A., Archer, B. N. and Heyderman, R. S. (2010). Typhoid fever and invasive nontyphoid salmonellosis, Malawi and South Africa. Emerg Infect Dis. 16: 1448–1451.
Khoharo, H. K., Ansari, S. and Qureshi, F. (2010). Evaluating single acutephase widal test for the diagnosis of typhoid fever. Med. Channel. 16(1): 42-44.
Kuhn, K. G., Falkenhorst, G., Ceper, T. H., Dalby, T., Ethelberg, S., Molbak, K., (2012). Detecting non-typhoid Salmonella in humans by ELISAs: A literature review. J. Med. Microbiol. 61(1):1–7.
Okike, N. and Ugwu, C. C. (2021). Isolation and identification of Salmonella sp and Shigella sp from different poultry feeds, droppings and drinking water used in poultry farms in Ishiagu, Ebonyi State. International Journal of Innovative Research and Advanced Studies (IJIRAS). 8: 2394-4404.
Salinas, L., Cárdenas, P., Johnson, T. J., Vasco, K., Graham, J. and Trueba, G. (2019). Diverse commensal Escherichia coli clones and plasmids disseminate antimicrobial resistance genes in domestic animals and children in a semirural community in Ecuador. mSphere. 4(3): 316–318.
Tiwaskar, M. (2019). Cefixime-ofloxacin Combination in the Management of Uncomplicated Typhoid Fever in the Indian Community Setting. J Assoc. Physicians India. 67(3): 75-80.
