ANTIOXIDANT AND ANTIHYPERGLYCEMIC PROPERTIES OF STERCULIA SETIGERA ROOT BARK EXTRACT ON ALLOXAN MONOHYDRATE-INDUCED WISTAR RATS
Abstract
Diabetes has racked serious havoc on human lives, and efforts to find a cure/remedy have extended to the use of traditional materials, especially from the plant kingdom. Therefore, the present study investigated the antioxidant and antihyperglycemic properties of S. setigera root bark. The plant material was obtained from the wild and gave a good yield of extract (25.12%) through cold maceration. Thirty Wistar rats weighing between 70 and 140 g were used in the antihyperglycemic study. The animals were divided into six groups of five rats per group. 25 rats were made diabetic through intraperitoneal (i.p) injection with 120 mg/kg of 150 mg alloxan monohydrate constituted in normal saline. Three groups of diabetic rats were treated oragastically with the extract 300 mg/kg, 750 mg/kg, and 1000 mg/kg). One group was left untreated and served as the diabetic control; the other was treated with the standard drug glibenclimide. The results showed a significant (p > 0.005) reduction in blood glucose level compared with the diabetic control. The extract was also active against ABTS and DPPH oxidants and reversed lipid parameters associated with diabetes. This indicated that the extract could be a potent drug source for the management of diabetes mellitus.
Keywords:
Sterculia setigera, Kumenduul, antioxidant, alloxan, antihyperglycemic, phytochemicalsDownloads
Published
DOI:
https://doi.org/10.5281/zenodo.17426565Issue
Section
How to Cite
License
Copyright (c) 2025 NengeHile Paschal, Iorsase Sarah , Jamil GarbaAbubakar
This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.
References
Adjanshoun, E., Ahyi, M. R. A., Ake-Assi, L., Elewude, J. I., Dramane, K., Fadoju, S. O., Gbile, Z. O., Goudole, E., Johnson, C. I. A., Keita, A., Morakinyo, O., Ojewole, J. A. O., Olatunj, A. O. and Sofowora, E. A. (1991). Traditional Medicine and Pharmacopia. Contribution to Ethanobotanical Floristic Studies in Western Nigeria, Pub. Organization of African Unity, scientific technical and research Commission Lagos, Nigeria. 420 pages.
Akah, J. A., Lemji, J. A., Salawa, O. A., Okeye, T. C. and Offiah, N. V. (2009). Effect of vernoniaamygdalina on biochemical and hematological parameters in rats with diabetes. Asian Journal of Medicinal Science, 13: 108-113
Atakpama, W., Batawila, K., Dourma, M., Pereki, H., Wala, K., Dimobe, K., Akpagana, K. and Gbeassor, M. (2012). Ethnobotanical Knowledge of Sterculiasetigera Del. in the Sudanian Zone of Togo, West Africa Hindawi, 2012. https://doi.org/10.5402/2012/723157.
Batum, N. (2006). Niumtrinobatumlinn. African Journal of Biotechnology, 5(23): 2402–2404
Blois, M. S. (1958). Antioxidant determinations by the use of a stable free radical nature, J BiolChem 181 1199–1200.
Belem, B., Nacoulma, B.M.I., Gbangou, R., Kambou, S., Hansen, H.H., Gausset, Q., Lund, S., Raebild, A., Lompo, D., Ouedraogo, M., Thielade & Boussim, I.J. (2007). Use of non-wood forest products by local people bordering the “Parc National KaboréTambi,” Burkina Faso. Journal of Transdisciplinary Environmental Studies, 6(1): 1–21.
Betti, L. J., S. R.MebereYemefa’a and F. NchembiTarla. 2011. Contribution to the knowledge of non-wood forest products in the far north region of Cameroon: Medicinal plants sold in the Kousséri market. Journal of Ecology and the Natural Environment 3(7):241–254.
Cennet, G. and Ebubekir, E. (2010). Experimental animal models of diabetes mellitusInternational Journal of Advances in Biological Sciences, 2(1), 1-7.
European Treaty Series (2005). European Convention for the Protection of Vertebrate Animals and Other Scientific Purposes-ETF-123
Frie, B. (1995). Cardiovascular disease and nutrient antioxidants: role of low-density lipoprotein oxidation. Journal Critical Review Food Science Nutrition, 35: 83-98.
Friedewald, W. T., Levy, R. T. and Frederickson, D. S. (1972). Estimation of low-density lipoprotein cholesterol concentration in plasma without the use of the preparative ultracentrifuge. Journal of Clinical Chemistry 18: 499-502.
Hasty, A. H., Shimano, H., Osuga, J., Namatame, I., Takahashi, A. & Yahagi, N. (2001). Severe hypercholesterolemia, hypertriglyceridemia, and atherosclerosis in mice lacking both leptin and lowdensity lipoprotein receptor.Journal of Biological Chemistry, 276: 37402-37408.
Idris, M. M. & Nenge, H. P. (2021).Free Radical Scavenging Activity and Histological Properties of Ethanol Extract of CrossopteryxFebrifugaStem Bark in Rats with Alloxan-Induced Diabetes. Global Scientific Journal, 9(9): 442- 454.
Igoli, J. O., Ogaji, O. U., Tor-Anyiin, T. A. and Igoli, N. P. (2005). Traditional Medicine Practices among the Igede People of Nigeria Part II. African Journal of Traditional, Complementary, and Alternative Medicine, 2(2), 134-152.
Kelly, W. (2023). A review of diabetes therapies and lifestyle changes. Medical news today, Healthline media uk. Available from: https://www.medicalnewstoday.com
Liu, R. H. (2003). The health benefits of fruits and vegetables are derived from the additive and synergistic combination of phytochemicals. American Journal of Clinical Nutrition, 78(3): 517-520.
Lorke, D. (1983). A New Approach to Practical Acute Toxicity Testing Archives of Toxicology: 542: pp: 75-287.
Mahmoud, B., Arman, Z., & Mahmoud, R. Kourosh, S. (2014). Ethnobotanical study of medicinal plants used in the management of diabetes mellitus in Urmia, northwest Iran. Asian Pacific Journal of Tropical Medicine. 7(1), pp: 348-354.
Manisha, W. H.,Richa, R. and Deepali, J. (2017). Oxidative Stress and Antioxidants: An Overview. International Journal of Advanced Research and Review, Vol. 2, No. 9, pp: 110-119.
Maury, G.L., Rodríguez, D.M., Hendrix, S., Arranz, J.C.E., Boix, Y.F., Pacheco, A.O., Díaz, J.G., Morris-Quevedo, H.J., Dubois, A.F., Aleman, E.I., Beenaerts, N., Méndez-Santos, I.E., Ratón, T.O., Cos, P. and Cuypers, A. (2020). Antioxidants in Plants: A Valorization Potential Emphasizing the Conservation of Plant Biodiversity in Cuba. Antioxidants (Basel), 27;9(11):1048. doi: 10.3390/antiox9111048.
Nisha, K. (2016). Diabetes Mellitus: Classification, Symptoms and Management: A Review. Res Rev Biol. 2014; 4:1-16.
Otang, W. M., Grierson, D. S. & Ndip, R. (2012). Phytochemical Studies and Antioxidant Activity of Two South African Medicinal Plants. Complementary and Alternative medicine. https://doi.org/10.1186/1472-6882-12-43
Piero, M. N., Nzaro, G. M. and Njagi, J. M. (2014). Diabetes- a Devastating Metabolic Disorder. Asian Journal of Biomedical and Pharmaceutical Sciences, 4(40): 1-7. DoI: 10.15272/ajbps.v40.645.
Re, R., Pellegrini, N., Proteggente, A., Pannala, A., Yang, M. and Riceevans, C. (1999). Applying an Improved ABTS Radical Decolorization Assay to Antioxidant Activity. Free Radical Biology and Medicine 26: 1231–1237.
Sicree, R., & Shaw, J. Zimmet, P. (2006). Global Burden of Diabetes and Impaired Glucose Tolerance: Prevalence and Projections Diabetes Education. Diabetes Atlas, 3rded. International Diabetes Federation, pp. 16-103.
Stein, E. A. (1987). Lipids, lipoproteins and apolipoproteins. In: N.W. Treitz, (Ed), Fundamentals of Clinical Chemistry.3rd Edition. W.B. Sauders, Philadelphia, pp: 470-479
Suhaj, M. (2006). Isolation of spice antioxidants and their antiradical activity: a review.Journal of Food Composition and Analysis 19: 531–537.
Switi, B. G., Krishna, M. G., & Sandhya, R. M. (2014). Phytochemicals for the Management of Diabetes.Pharmaceutical Crops, 5 (Suppl 1: M2): 11-28 11.
Tadhani, M. B., Patel, V. H. and Subhash, R. (2007). In vitro antioxidant activity of Steviarebadiana leaves and callusca. Journal of Food Composition and Analysis, 20: 323–329.
Tchoubou, Z., Koubala, B. B. & Ndjonka, D. (2023). Antioxidant Potential and Insulin Resistance Improvement in Rats with Type 2 Diabetes Induced byHigh-Fat Diet and Low Streptozotocin Dose. Dose-Response:An International Journal, 1–12, https://doi.org/10.1177/15593258231185460
Tietz, N. W. (1990). Serum TG Determination In: Clinical Guide to Laboratory Tests, Second Edition, W.B, Saunders Co, Philadelphia USA. 554-556.
Tor-Anyiin, T. A., Akpuaka, M. U. and Oluma, H. O. A. (2011). Phytochemical and antimicrobial studies on SterculiasetigeraDelile stem bark. African Journal of Biotechnology, 10(53): 11011–11015.
Ujah, F.O., Berinyuy, E.B., Edeh, F.O. and Ujah, R.I (2015). Phytochemical Screening and Combined Effect of Ethanolic Leaf Extracts of Phyllanthusamarus and Psidium guajava in Rats with Streptozotocin-Induced Diabetes. Asian Journal of Biochemical and Pharmaceutical Research, 2(5): 211-223.
Vankata, R. V., Kumar, P. O., SivanageswararaoMandSrinivasu, K. (2016). Appraisal of Antimicrobial Medicinal Plants with Potential Therapeutic Effect on Diabetes and Sickle Cell Traits. Asian Journal of Phytomedicine and Clinical Research, 4(2), 57-68, p. 68.
Wacnic, R. G.,&Alber, J. J.(1978). Comprehensive evaluation of the heparin manganese precipitation procedure for estimating cholesterol levels of high density lipoproteins. Journal of Lipid Research, 19: 65-76.
