International Research Journal of Medical and Pharmaceutical Sciences (IRJMPS)

NEURODEGENRATION AND INFLAMATORY EFFECTS OF CONCURRENT USE OF CAFFEINE AND ALCOHOL ON HIPPOCAMPAL CA1 IN WISTAR RATS (Rattus novergicus).

Authors

  • Adeleye Olufunto Omodele Department of Anatomy, Faculty of Basic Medical Sciences, Ladoke Akintola University of Technology, Nigeria.
  • Oyawaluya Bamisaye Olaofe Department of Pharmaceutical Chemistry, Faculty of Pharmacy, University of Lagos, Nigeria.
  • Yusuf Joshua Ayodele Department of Molecular Biology and Biotechnology, Nigerian Institute of Medical Research, Nigeria.
  • Adelakin lola Adeola4 Department of Anatomy, Faculty of Basic Medical Sciences, Babcock University, Nigeria.
  • Adejobi Emmanuel Oluwagbenga Department of Anatomy, Faculty of Basic Medical Sciences, Ladoke Akintola University of Technology, Nigeria.

Abstract

A lot has been said about the neuroprotective effects of caffeine on neurodegenerative diseases at low dosage, but little has been said about its neurotoxic effects, especially when consumed with alcohol, on the CA1 region of the brain that is responsible for learning and memory and has been reported to be sensitive to neurotoxic insults. Therefore, this study aimed to determine the neurodegeneration and inflammatory effects of caffeine-alcohol consumption on the CA1 region of the hippocampus in male Wistar rats.

A total of twenty (20) adult Wistar rats weighing 120-140g were used in this study. The following experimental groups were designed for the study: control were given distilled water; caffeine-treated group were administered with 10 mg/kg/bw, Alcohol-treated group were administered with 30%v/v of alcohol; and the caffeine plus alcohol-treated group took 10 mg/kg/bw of caffeine plus 30%v/v of alcohol) and the administration were done for 30days after which the animals were euthanized using 0.1ml Ketamine Hydrochloride (intraperitoneally administered, then transcardiac perfusion was done using 0.9% Normal saline for about 2 minutes following 10% Neutral Buffered Formalin (NBF). The regions of the hippocampus were dissected out, weighed, and fixed in 10% Formol Saline for 24/48 hr.

Keywords:

Alcohol, Caffeine, Inflammation, Neurodegeneration,, Hippocampus

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Published

2024-06-17

DOI:

https://doi.org/10.5281/zenodo.11917220

Issue

Vol. 9 No. 2 (2024): April-June

Section

Articles

How to Cite

Adeleye , O. O., Oyawaluya , B. O., Ayodele, Y. J., Adelakin lola Adeola4, & Adejobi Emmanuel Oluwagbenga. (2024). NEURODEGENRATION AND INFLAMATORY EFFECTS OF CONCURRENT USE OF CAFFEINE AND ALCOHOL ON HIPPOCAMPAL CA1 IN WISTAR RATS (Rattus novergicus). International Research Journal of Medical and Pharmaceutical Sciences (IRJMPS), 9(2), 38–52. https://doi.org/10.5281/zenodo.11917220

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Copyright (c) 2024 Adeleye Olufunto Omodele, Oyawaluya Bamisaye Olaofe, Yusuf Joshua Ayodele, Adejobi Emmanuel Oluwagbenga, Adelakin lola Adeola4

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This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

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