Advanced Journal of Environmental Sciences (AJES)

EFFECT OF PETROLEUM PRODUCT INHALATION ON THE HISTOLOGICAL INDICES OF THE LUNGS OF MALE WISTAR RATS

Authors

  • Offor Amarachukwu Grace Department of Applied Biology and Biotechnology, Enugu State University of Science and Technology (ESUT), Enugu Nigeria.
  • Edoga Cyril Onyekachi Department of Applied Biology and Biotechnology, Enugu State University of Science and Technology (ESUT), Enugu Nigeria.
  • Okeke, Chukwuemeka Christian Department of Applied Biology and Biotechnology, Enugu State University of Science and Technology (ESUT), Enugu Nigeria.

Abstract

Petroleum product exposure is a known risk factor for respiratory diseases. This study investigated the effect of petroleum product inhalation on oxidative stress parameters and histopathological indices of the lung tissue of male Wistar albino rats. Forty-five rats aged 3 months and weighing 180–220 g were divided into five groups: Group A (Control), Group B (PMS exposure - 500 ml), Group C (Kerosene exposure - 500 ml), Group D (Diesel exposure - 500 ml), and Group E (Mixed petroleum products - 500 ml). The exposure lasted for 28 days, and lung tissue homogenate samples were collected at days 0, 14, and 28 to measure superoxide dismutase (SOD), catalase (CAT) activity and glutathione-s-transferase (GST) activity. The lungs were harvested using dissection to test the histological effects of the exposure. The results showed that SOD and CAT activity were significantly decreased (p<0.05) (11.12 ± 0.48 and 1.63 ± 0.23) when compared with the control (12.87 ± 0.93 and 3.29 ± 0.70) while GST activity significantly increased (p>0.05) (24.02 ± 0.54) compared with the control (23.20 ± 0.45). Histology of the lungs showing degeneration of the dentate granular layer in male Wistar albino rats exposed to diesel. These results emphasize the harmful effects of inhaling petroleum products on critical organ systems, particularly the lungs.

Author Biographies

Offor Amarachukwu Grace, Department of Applied Biology and Biotechnology, Enugu State University of Science and Technology (ESUT), Enugu Nigeria.

Offor, Amarachukwu Grace, hails from Umudia in Igbo-Etiti Local Government Area of Enugu State, Nigeria. I began my academic journey at Ideal Schools and College in Saki West Local Government, Oyo State, where I completed my primary education. She continued her secondary education at Benefit Comprehensive College in the same area. I obtained B.Sc. and M.Sc. degrees from Enugu State University of Science and Technology (ESUT). My academic and research pursuits are focused on environmental biology and ecotoxicology, where she is passionate about studying the impact of pollutants on ecosystems and contributing to environmental sustainability

Edoga Cyril Onyekachi , Department of Applied Biology and Biotechnology, Enugu State University of Science and Technology (ESUT), Enugu Nigeria.

Dr. Cyril Onyekachi Edoga was born on 2nd August, 1984 in Mgboko Aku, Igbo Etiti LGA of Enugu State, Nigeria. My academic journey led me to earn a Ph.D. in Animal and Environmental Physiology from the University of Nigeria, Nsukka. Currently a senior academic at Enugu State University of Science and Technology (ESUT). In 2024, I was conferred with Chieftaincy title as Ebekuedike 1 of Ukwuoto Edenu Ede-Oballa Ancient kingdom. As a devout Catholic, I integrate faith into my leadership, striving to uplift my people through education and moral guidance. My work bridges academia, research, community development and spirituality, dedicated to the service of God and humanity

Okeke, Chukwuemeka Christian, Department of Applied Biology and Biotechnology, Enugu State University of Science and Technology (ESUT), Enugu Nigeria.

Okeke, Christian Chukwuemeka, is proudly from Umuakwu in Njikoka Local Government Area of Anambra State, Nigeria. He did his primary education in De Women’s Corona Society Primary School in Jos South, Plateau State, Nigeria. He went further to do his secondary education in St. Joseph Secondary School in Jos South, Plateau State, Nigeria. He furthered his B.Sc. in Enugu State University of Science and Technology, where he studied Applied Biology and Biotechnology. He is keen about research that is targeted at solving problems of man and his environment

Keywords:

Superoxide Dismutase, Catalase, Petroleum products, Lungs, Histology

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Published

2025-04-24

DOI:

https://doi.org/10.5281/zenodo.15274225

Issue

Vol. 16 No. 4 (2025): April

Section

Articles

How to Cite

Offor, A. G., Edoga, C. O., & Okeke, C. C. (2025). EFFECT OF PETROLEUM PRODUCT INHALATION ON THE HISTOLOGICAL INDICES OF THE LUNGS OF MALE WISTAR RATS. Advanced Journal of Environmental Sciences (AJES), 16(4), 19–30. https://doi.org/10.5281/zenodo.15274225

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Copyright (c) 2025 Offor Amarachukwu Grace, Edoga Cyril Onyekachi , Okeke, Chukwuemeka Christian

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This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

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