International Research Journal of Medical and Pharmaceutical Sciences (IRJMPS)

THEOBROMA CACAO SEED EXTRACT ATTENUATES RESERPINE-INDUCED HEMATOTOXICITY AND STOMACH TISSUE DISTORTIONS IN MALE WISTAR RATS

Authors

  • Adetunji Opeyemi Adebola Department of Anatomy, School of Basic Medical Sciences, Benjamin S. Carson (Snr.) College of Health and Medical Sciences, Babcock University, Ogun State, Nigeria
  • Adeoye Bayo Olufunso Department of Biochemistry, School of Basic Medical Sciences, Benjamin S. Carson (Snr.) College of Health and Medical Sciences, Babcock University, Ogun State, Nigeria
  • Akano Oyedayo Phillips Department of Physiology, School of Basic Medical Sciences, Benjamin S. Carson (Snr.) College of Health and Medical Sciences, Babcock University, Ogun State, Nigeria
  • Adeoye Ayodeji David Department of Physiology, School of Basic Medical Sciences, Benjamin S. Carson (Snr.) College of Health and Medical Sciences, Babcock University, Ogun State, Nigeria
  • Adeogun Adetomiwa Ezekiel Department of Physiology, School of Basic Medical Sciences, Benjamin S. Carson (Snr.) College of Health and Medical Sciences, Babcock University, Ogun State, Nigeria
  • Adebayo Iyanuoluwa Oluwadunsin Department of Physiology, School of Basic Medical Sciences, Benjamin S. Carson (Snr.) College of Health and Medical Sciences, Babcock University, Ogun State, Nigeria
  • Fabiyi Oluseyi Sunday Department of Anatomy, School of Basic Medical Sciences, Benjamin S. Carson (Snr.) College of Health and Medical Sciences, Babcock University, Ogun State, Nigeria
  • Ogunsanya Sanmi Tunde Department of Anatomy, School of Basic Medical Sciences, Benjamin S. Carson (Snr.) College of Health and Medical Sciences, Babcock University, Ogun State, Nigeria
  • Nwobi Nnenna Linda Department of Chemical Pathology, Faculty of Basic Clinical Sciences, Benjamin S. Carson (Snr.) College of Health and Medical Sciences, Babcock University, Ogun State, Nigeria.
  • Nwobi Joseph Chigbogu Department of Biochemistry, School of Basic Medical Sciences, Benjamin S. Carson (Snr.) College of Health and Medical Sciences, Babcock University, Ogun State, Nigeria
  • Osundina Oluwaseun Babatunde Department of Biochemistry, Osun State University, Osogbo, Osun State, Nigeria
  • Achor Corniluis Bangsi Department of Biochemistry, School of Basic Medical Sciences, Benjamin S. Carson (Snr.) College of Health and Medical Sciences, Babcock University, Ogun State, Nigeria.
  • Olatinwo Goodness Olusayo, Department of Physiology, School of Basic Medical Sciences, Benjamin S. Carson (Snr.) College of Health and Medical Sciences, Babcock University, Ogun State, Nigeria
  • Kolawole Oluwaseyi Emmanuel Department of Anatomy, School of Basic Medical Sciences, Benjamin S. Carson (Snr.) College of Health and Medical Sciences, Babcock University, Ogun State, Nigeria
  • Lawal Ismail Adetayo Department of Anatomy, Al-Hikmah University, Ilorin, Kwara State, Nigeria
  • Adebayo Barakat Temitope Department of Biochemistry, Ladoke Akintola University of Technology, Ogbomoso, Oyo State, Nigeria
  • Ademoye Kehinde Aderonke Department of Physiological Sciences, Obafemi Awolowo University, Ile-Ife, Osun-State, Nigeria

Abstract

Alterations in blood cells and gastrointestinal (GIT) parameters are intertwined with various physiological, psychological, and environmental factors. This research elucidated the potential pharmacological roles of Theobroma cacao seed ethanolic extract on blood profile and stomach tissue aberration induced by reserpine. A total of thirty (30) adult male Wistar rats were used for this study for a period of 28 days and were divided into six groups as follows: Group A (control group), Group B (0.5mg/kg/ B.W of reserpine), Group C (20mg/kg/B.W fluoxetine), Group D (1000mg/kg/B.W of cocoa seed ethanolic extract), Group E (0.5mg/kg/B.W of reserpine + 1000mg/kg/B.W of cocoa seed ethanolic extract), and Group F (0.5mg/kg/B.W of reserpine + 20mg/kg/B.W of fluoxetine). The stomach tissue was harvested, placed in 10% neutral buffered formalin, and processed for histological analysis. A full blood count test was performed, and the results were analyzed using graph pad prism 9.0 software using one-way analysis of variance (ANOVA). The results showed a decrease in PCV (packed cell volume), hemoglobin, and neutrophils of the reserpine-induced rats. There was a significant increase in the treated groups. Histopathology assessments depicted that treatment with Theobroma cacao seed ethanolic extract groups restored mucosa thickness and curtailed glycogen accumulation in the stomach tissue. This study showed that the ameliorative effect of cocoa seed ethanolic extract is pronounced in reversing the harmful effects of reserpine on blood profiles and GIT

Keywords:

Reserpine, Gastrointestinal tract, Theobroma Cacao, Hematotoxicity, Full Blood Count

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Published

2024-05-31

DOI:

https://doi.org/10.5281/zenodo.11402037

Issue

Vol. 9 No. 2 (2024): April-June

Section

Articles

How to Cite

Adetunji , O. A., Adeoye , B. O., Akano , O. P., Adeoye , A. D., Adeogun , A. E., Adebayo , I. O., … Ademoye , K. A. (2024). THEOBROMA CACAO SEED EXTRACT ATTENUATES RESERPINE-INDUCED HEMATOTOXICITY AND STOMACH TISSUE DISTORTIONS IN MALE WISTAR RATS. International Research Journal of Medical and Pharmaceutical Sciences (IRJMPS), 9(2), 23–37. https://doi.org/10.5281/zenodo.11402037

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Copyright (c) 2024 Adetunji Opeyemi Adebola, Adeoye Bayo Olufunso, Akano Oyedayo Phillips, Adeoye Ayodeji David, Adeogun Adetomiwa Ezekiel, Adebayo Iyanuoluwa Oluwadunsin, Fabiyi Oluseyi Sunday, Ogunsanya Sanmi Tunde, Nwobi Nnenna Linda, Nwobi Joseph Chigbogu, Osundina Oluwaseun Babatunde, Achor Corniluis Bangsi, Olatinwo Goodness Olusayo, , Kolawole Oluwaseyi Emmanuel, Lawal Ismail Adetayo, Adebayo Barakat Temitope, Ademoye Kehinde Aderonke

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This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

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